Altere a cor do botão em pairando tknter
import tkinter as tk
def on_enter(e):
myButton['background'] = 'green'
def on_leave(e):
myButton['background'] = 'SystemButtonFace'
root = tk.Tk()
myButton = tk.Button(root,text="Click Me")
myButton.grid()
myButton.bind("<Enter>", on_enter)
myButton.bind("<Leave>", on_leave)
root.mainloop()
Tired Tuatara